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KMID : 1035420210090010163
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Journal of The Korean Society of Integrative Medicine
2021 Volume.9 No. 1 p.163 ~ p.171
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The Effect of Water Extracted from Sparassis crispa on the Expression of TNF-α, iNOS and IL-1β Genes in RAW 264.7 Cells
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Han Hyo-Sang
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Abstract
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Purpose: The purpose of this study was to examine the anti-inflammatory effects of Sparassis crispa (SC). SC is a well-known traditional herbal remedy and its mushroom is used for treatment of inflammation. Many diseases that are increasing recently have characteristics of inflammatory diseases. Researchers are finding bioactive substances from natural products that can promote treatment and prevention of inflammation. We investigated the effect of water extracted from SC on the expression of effector genes involved in the function of RAW 264.7 cells.
Methods: Effects of RAW 264.7 cells on cell viability, antioxidation, and mRNA expression were examined using water extracts from SC. A 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to determine the effect of water extracts from SC on cell viability in RAW 264.7 cells. Inflammation of RAW 264.7 cells induced by lipopolysaccharide (LPS) treatment and expression levels of inflammatory cytokine TNF-α, iNOS and IL-1β gene were analyzed using quantitative reverse transcription PCR (qRT-PCR) analysis.
Results: The MTS assay was performed on RAW 264.7 cells after treatment with various concentrations of water extracts of SC. Treatment of RAW 264.7 cells with water extracts from LPS and SC at a concentration of 0.125, 0.5 ㎎/㎖ for twentyfour hours promoted mRNA expression of TNF-α, iNOS and IL-1β.
Conclusion: MTS assay was applied to RAW 264.7 cells after various concentrations of water extracts of SC. Through experimental demonstration of anti-oxidant and anti-inflammatory effects of water extracts from SC, we suggest that SC is a valuable material for the prevention and treatment of various inflammatory diseases.
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KEYWORD
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anti-inflammation, lipopolysaccharide, MTS assay, RAW 264.7 cell, Sparassis crispa
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